Resources

  • Research Technician

    The laboratory of Professor Harris Wang in the Department of Systems Biology is seeking a research Technician B. The work in the Wang laboratory is focused on diverse questions in systems and synthetic biology including mammalian microbiome function, host-microbiome interactions in health and disease, microbial horizontal gene flow, and foundational technology development. The candidate for this position will carry out routine molecular biology and microbiology experiments, microbiome studies in vitro and in animal models, library preparations for next-generation sequencing, and microbial cultivation studies to serve ongoing projects.

  • Goverment Project Administrator

    Company Overview:

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core valuesThis position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    Position Summary:

    Project monitoring and management support for LanzaTech’s portfolio of pioneering, technologically complex, multi-year projects that receive funding from government and other partners
    Support Government team leadership in ensuring successful project execution and reporting
    Directly interface with major stakeholders including internal technical team and project leads, government funding project monitors, as well as counterparts at sub-contractor and partner organizations

    Specific Roles:

    Project monitoring and management of government funded projects, including:

    Support project teams during project initiation and implementation
    Coordinate and implement workflows to facilitate project technical and financial objectives
    Monitor project performance against awarded cost, schedule, and milestones
    Coordinate and prepare written reports on project status including scheduling and tracking of progress and deliverables
    Design and prepare status summaries for Government team leadership

    Qualifications:

    BA or BS Degree required
    3+ years of experience monitoring and management of scientific research and/or engineering projects required
    Experience executing and/or managing government funded project is preferred.
    High proficiency with Microsoft Office required; experience with SharePoint preferred
    Demonstrated ability to work in a team environment
    Excellent data gathering and organization skills
    Excellent verbal and written communication skills

    Work Conditions:

    Able to work in a general office and desk environment.
    Sitting and working at a desk for long durations.
    Working on a computer for long durations.

  • Lead Scientist, Cell-Free

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    LanzaTech is looking for a motivated, high energy, well-organized, collaborative Scientist to lead research and development of cell-free technologies at LanzaTech. The role will include:

    Work on a cross-functional team to support projects that require and/or would benefit from cell-free applications and experiments
    Leading the development, optimization, and automation of cell-free protein synthesis workflows both in model systems and gas-fermenting organisms
    Evaluating and leading testing of additional cell-free applications such as biosensors and manufacturing
    Conducting work collaboratively across multiple teams at LanzaTech including Automation, Fermentation, Analytics, and Engineering

    Responsibilities

    Follow all safety requirements for working in the offices and laboratories, including completing any required trainings
    Maintain a clean and functional laboratory space
    Assist in upkeep of laboratory reagents and stock supplies to run experiments
    Maintain an up-to-date laboratory notebook tracking experiments, data, and interpretation of results
    Perform cell-free experiments and workflows in support of externally funded projects
    Lead the development of new cell-free workflows and capabilities at LanzaTech
    Establish and write standard operating procedures (SOPs) for laboratory workflows
    Stay up-to-date with advancements in cell-free scientific field
    Attend and participate in Enzymology and Synthetic Biology group meetings as well as relevant project updates with partners
    Present findings and developments both internally to LanzaTech teams and externally to partners
    Instruct and advise LanzaTech colleagues on established workflows
    Guide and train junior scientists such as research associates, technicians, and interns
    Develop research plans and preliminary data in support of grant proposals
    Use of laboratory management systems, in-house experimental design tools, and computational tools to improve efficiency in design, execution, and interpretation of experiments

    Requirements

    Minimum

    Ph.D. in Biochemistry, Synthetic Biology, Bioengineering, Molecular Biology, Chemical Engineering, or a related field OR M.Sc. in related field with 2+ years relevant laboratory experience with cell-free applications post-graduation
    Demonstrated experience with designing, executing, and reporting cell-free experiments
    Knowledgeable with the current cell-free protein synthesis/cell-free applications field
    Strong organizational, time management, and interpersonal skills
    Experience writing reports and/or manuscripts centered on cell-free applications (e.g., transcription, translation, sensing and screening)
    Experience giving group presentations
    High attention to detail and careful record keeping
    Independent and highly self-motivated
    Excellent communication skills and a desire to work in a team
    Ability to conduct research independently and manage multiple tasks in parallel
    Awareness of safety issues in the work environment
    Strong computer literacy including Microsoft Office, SharePoint or equivalent applications

    Highly Preferred

    Experience and/or working knowledge of anaerobic microbes
    Experience with multiple cell-free protein synthesis systems (e.g., E. coli and Wheat Germ)
    Experience with multiple protein reporter, detection, and/or quantification approaches (e.g., luciferase, radiolabeling, and blotting)
    Experience with multiple DNA cloning methods (e.g., Gibson and Golden Gate assemblies)

    Location

    LanzaTech Headquarters
    Skokie, IL USA

    Apply HERE

  • Scientist, Automation Scientist

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    We are seeking an Automation Scientist to, develop automated and high throughput method for a number of tasks upstream and downstream of LanzaTech BioFoundry LanzaTech has designed, built and operates the world-first anaerobic biofoundry, a fully integrated platform of several automated modules with the capability of growing and manipulating thousands of anaerobic strains on gaseous feedstocks. A successful candidate will join LanzaTech’s Automation team and be a part of an effort pushing the boundaries of automation in context of anaerobic conditions and non-model organisms. The Automation team is a cross-functional team within LanzaTech’s Synthetic Biology group who support a variety of projects both within the group and across the company.

    Activities include Will primarily involve assisting not only the automation team in developing new workflows but also other team within the company that has automation amenable workflows. A successful candidate will also be expected to participate in data capture through both automated and semi-automated data management systems and the daily execution of high-throughput experiments and method development and optimization as well as coordination with various project teams.

    Duties & Responsibilities:

    Developing automated workflows for a number of teams within lanzatech
    Daily operations of developed workflows and general automation team work
    Definition, development and optimization of high-throughput workflows including integration of new instruments, emerging technologies and information systems;
    Use of laboratory information management systems (LIMS) and bioinformatics software tools and databases including data capture from high-throughput experiments and analysis of statistics and results against model predictions;
    Work in cross-functional setting including coordination with project team leaders;
    Attend seminars and participate in group and project meetings or lab activities as required.

    Requirements:

    M.Sc. or B.Sc. (or equivalent) in Synthetic Biology, Bioengineering, Biochemistry, Molecular Biology, Chemical Engineering or a related field.
    Minimum of 2 years’ experience in biotechnology industry, a biofoundry or a high-throughput environment with hand-on experience in high-throughput workflow development and/or execution, preferentially with high throughput microbial culture screening.
    Proficient in working with laboratory automation software and/or programming as well as statistics. Programming Hamilton liquid handing systems (Venus programming) and Thermo scheduling software (Momentum™) is highly preferred.
    Strong organizational, time management, and interpersonal skills. Ability to multi-task and keep track of multiple tasks and projects at the same time. High attention to detail and careful record keeping.
    Independent and highly self-motivated. Excellent communication skills and team-work oriented.
    Awareness of safety issues in the work environment.

    Desirable Skills:

    Proficiency in programming (e.g. Python)
    Experience with SQL databases and LIMS systems
    Experience with cell sorting
    Experience with microfluidics.
    Experience working with anaerobic microbes.

    Location

    LanzaTech Headquarters
    Skokie, IL USA

    Apply HERE

  • Senior Research Associate, CRISPR

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    We are seeking a Research Associate to develop and execute experiments leveraging CRISPR-based synthetic biology techniques towards engineering and characterization of LanzaTech’s proprietary gas fermenting microbes. The Research Associate will be joining LanzaTech’s Synthetic Biology team, working in the lab and interacting with other teams across the company. This role will support Senior Scientists on project-based work as well as a variety of lab management tasks. The Research Associate will work towards both specific goals and towards improving workflows to benefit all Synthetic Biology team members. Types of activities include plasmid construction, bacterial transformation and culturing, DNA QC, product screening, genetic parts characterization, curating plasmid repositories, preparing buffer and media, stocking consumables, authoring training and protocol documents, data analysis, ect. Overly qualified applications may be considered at

    Duties & Responsibilities:

    Design, assembly, and transformation of DNA constructs
    Genetic modification and selection of LanzaTech proprietary microbes
    High-throughput cultivation and screening of LanzaTech proprietary microbes
    Analysis of data and interpretation of results against model predictions
    Use of computational tools and databases
    Execute established workflows and implement improvements
    Support organization of training, protocol, and other document development
    Maintain a laboratory notebook, track and QC data in LIMS system as required
    Attend seminars and participate in group and project meetings and lab activities

    Requirements:

    M.Sc. or B.Sc. (or equivalent) in Synthetic Biology, Bioengineering, Molecular Biology, Chemical Engineering, Computational Biology, or a related field with preferable 1-2 years of relevant experience (RA) or 3-5 years (Senior RA) in a laboratory setting
    Experience in cloning and assembly of DNA and growth of microbes or experience with NGS statistical analysis and an interest in performing wet-lab biology
    Knowledge of CRISPR-Cas systems
    Use of word processing, data collaboration tools, presentation and spreadsheet applications, and familiarity with concepts in statistics and tools to analyze data
    Strong organizational, time management, record keeping, self and prioritization skills
    Independent, hard-working, forward thinking, and highly self-motivated
    Excellent communication and interpersonal relationship skills
    Desire to work in a team and assist other team members
    Awareness of safety issues in the work environment

    Highly preferred:

    Experience different cloning methods such as Gibson or Golden Gate assembly and genetic tools such as CRISPR and homologous recombination
    Experience working with DNA design software and analysis of NGS data
    Strong computer literacy, including Microsoft Excel, PowerPoint, and Outlook
    Experience with at least one programming language, such as but not limited to Python, Java, R, or other statistical analysis pipelines
    Ability to conduct research independently and manage multiple tasks in parallel
    Experience with gas fermentation or working with anaerobic systems
    Experience conducting, reporting, and presenting experiments

    Location

    LanzaTech Headquarters
    Skokie, IL USA

    Apply HERE

  • Research Associate, DNA Assembly

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    LanzaTech is looking for a motivated, high energy, well-organized, collaborative Research Associate to support its high throughput DNA assembly pipeline.

    This person will work on a cross-functional team to support projects with their DNA assembly needs
    The Research Associate will work with the Automation team to develop, execute and optimize DNA assembly methods
    They will conduct their work collaboratively across multiple projects and functional teams

    Responsibilities

    Follow all safety requirements for working in the offices and laboratories, including completing any required training
    Maintain a clean and functional laboratory space
    Assist in the upkeep of laboratory reagents and stock supplies to run experiments
    Enter and update data in LIMS systems
    Daily running and maintenance of LanzaTech’s DNA assembly automated platform
    Attend and participate in Automation and Synthetic Biology group meetings as well as relevant project updates with partners
    Present findings and developments both internally to LanzaTech teams and externally to partners
    Use of laboratory management systems, in-house experimental design tools, and computational tools to improve efficiency in design, execution, and interpretation of experiments
    Assist in both training and development support of DNA assembly-related software tools

    Requirements

    Minimum

    B.Sc. or M.Sc. in Biochemistry, Synthetic Biology, Bioengineering, Molecular Biology, Chemical Engineering, or a related field
    Knowledgeable with cutting edge DNA assembly methods
    Experience using automated DNA assembly methods and equipment
    Strong organizational, time management, and interpersonal skills
    Experience writing reports and manuscripts
    Experience giving oral presentations
    High attention to detail and careful record-keeping
    Independent and highly self-motivated
    Excellent communication skills and a desire to work collaboratively in a team
    Awareness of safety issues in the work environment
    Strong computer literacy with Microsoft Office, SharePoint, or equivalent applications

    Preferred

    User level experience with Hamilton Venus
    Experience with DNA CAD and LIMS systems
    Ability to conduct research independently and manage multiple tasks in parallel

    Location

    LanzaTech Headquarters
    Skokie, IL USA

    Apply HERE

  • Research Associate, Cell-Free

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    LanzaTech is looking for a motivated, high energy, well-organized, collaborative Research Associate to support research and development of cell-free technologies within the context of industrial gas-fermenting bacteria.

    This person will work on a cross-functional team to support projects that require and/or would benefit from cell-free applications and experiments
    The Research Associate will work with the Enzymology team to develop and execute various cell-free protein synthesis workflows both in model systems and gas-fermenting organisms
    They will also participate in evaluating and testing additional cell-free applications such as biosensors and manufacturing
    They will conduct their work collaboratively across multiple teams at LanzaTech including Synthetic Biology, Fermentation, and Analytics

    Responsibilities

    Follow all safety requirements for working in the offices and laboratories, including completing any required trainings
    Maintain a clean and functional laboratory space
    Assist in upkeep of laboratory reagents and stock supplies in order to run experiments
    Maintain an up-to-date laboratory notebook tracking experiments, data, and interpretation of results
    Perform cell-free experiments and workflows in support of externally funded projects
    Establish and write standard operating procedures (SOPs) for laboratory workflows
    Attend and participate in Enzymology and Synthetic Biology group meetings as well as relevant project updates with partners
    Present findings and developments both internally to LanzaTech teams and externally to partners
    Use of laboratory management systems, in-house experimental design tools, and computational tools to improve efficiency in design, execution, and interpretation of experiments

    Requirements

    Minimum

    B.Sc. or M.Sc. in Biochemistry, Synthetic Biology, Bioengineering, Molecular Biology, Chemical Engineering, or a related field
    Knowledgeable with a current cell-free protein synthesis/expression or other cell-free application
    Strong organizational, time management, and interpersonal skills
    Experience writing reports and/or manuscripts
    Experience giving oral presentations
    High attention to detail and careful record keeping
    Independent and highly self-motivated
    Excellent communication skills and a desire to work in a team
    Awareness of safety issues in the work environment
    Strong computer literacy with Microsoft Office, SharePoint, or equivalent applications

    Highly Preferred

    Direct experience with multiple cell-free protein synthesis applications
    Experience and/or working knowledge of anaerobic microbes
    Experience with protein reporter, detection, and/or quantification approaches (e.g., luciferase, radiolabeling, and blotting)
    Experience with DNA cloning methods (e.g., Gibson and Golden Gate assemblies)
    Ability to conduct research independently and manage multiple tasks in parallel

    Location

    LanzaTech Headquarters
    Skokie, IL USA

    Apply HERE

  • Research Associate, Metabolic Engineering

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    We are seeking a Research Associate to improve laboratory processes and support metabolic engineering and characterization of LanzaTech’s proprietary gas fermenting microbes. The Research Associate will be joining LanzaTech’s Synthetic Biology team, working in the lab and interacting with other teams across the company. This role will support Senior Scientists on project-based work as well as a variety of lab management tasks. The Research Associate will work towards both specific goals and towards improving workflows to benefit all Synthetic Biology team members. Types of activities include plasmid construction, bacterial transformation and culturing, confirmation of clones, product screening, genetic parts characterization, curating strain and plasmid repositories, preparing buffer and media, stocking consumables, authoring training and protocol documents, etc.

    Duties & Responsibilities:

    Design, cloning and assembly of multi-fragment DNA constructs for heterologous pathway expression
    Genetic modification and selection of LanzaTech proprietary microbes
    High-throughput cultivation and screening of LanzaTech proprietary microbes
    Analysis of data and interpretation of results against model predictions
    Use of computational tools and databases
    Learn established workflows and implement improvements
    Support organization of training, protocol, and other document development
    Assist in general lab chores such as solution prep, cleaning, organization, etc.
    Maintain a laboratory notebook, track and QC data in LIMS system as required
    Attend seminars and participate in group and project meetings and lab activities

    Requirements:

    M.Sc. or B.Sc. (or equivalent) in Synthetic Biology, Bioengineering, Biochemistry, Molecular Biology, Chemical Engineering or a related field with preferable 1-2 years of relevant experience in a laboratory setting
    Hands-on experience in cloning and assembly of DNA and growth of microbes
    Use of DNA design software, word processing, data collaboration tools, presentation and spreadsheet applications, and familiarity with concepts in statistics and tools to analyze data
    Strong organizational, time management, record keeping, and prioritization skills
    Independent, hard-working, and highly self-motivated
    Excellent communication and interpersonal relationship skills
    Ability to maintain a positive attitude when faced with challenges
    Desire to work in a team and assist other team members
    Awareness of safety issues in the work environment

    Highly preferred:

    Experience with gas fermentation
    Experience in biotech industry
    Experience with metabolic engineering
    Experience different cloning methods such as Gibson or Golden Gate assembly and genetic tools such as CRISPR and homologous recombination
    Experience working with genetic and metabolic pathway databases and DNA design software
    Experience working with anaerobic microbes
    Experience working with automated liquid handler
    Strong computer literacy, including Office, SharePoint; Experience in scripting and working with large datasets is preferred
    Ability to conduct research independently and manage multiple tasks in parallel

    Location

    LanzaTech Headquarters
    Skokie, IL USA

     

    Apply HERE

  • Research Associate, Synthetic Biology

    About LanzaTech

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2019 Chicago Innovation Awards Winner
    • 2019 CNBC #15 on Top 50 Disrupter Companies
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the role

    We are seeking a Research Associate to improve laboratory processes and support metabolic engineering and characterization of LanzaTech’s proprietary gas fermenting microbes. The Research Associate will be joining LanzaTech’s Synthetic Biology team, working in the lab and interacting with other teams across the company. This role will support Senior Scientists on project-based work as well as a variety of lab management tasks. The Research Associate will work towards both specific goals and towards improving workflows to benefit all Synthetic Biology team members. Types of activities include plasmid construction, bacterial transformation and culturing, confirmation of clones, product screening, genetic parts characterization, curating strain and plasmid repositories, preparing buffer and media, stocking consumables, authoring training and protocol documents, etc.

    Key duties

    Design, cloning and assembly of multi-fragment DNA constructs for heterologous pathway expression
    Genetic modification and selection of LanzaTech proprietary microbes
    High-throughput cultivation and screening of LanzaTech proprietary microbes
    Analysis of data and interpretation of results against model predictions
    Use of computational tools and databases
    Learn established workflows and implement improvements
    Support organization of training, protocol, and other document development
    Assist in general lab chores such as solution prep, cleaning, organization, etc.
    Maintain a laboratory notebook, track and QC data in LIMS system as required
    Attend seminars and participate in group and project meetings and lab activities

    Required Qualifications

    M.Sc. or B.Sc. (or equivalent) in Synthetic Biology, Bioengineering, Biochemistry, Molecular Biology, Chemical Engineering or a related field with preferable 1-2 years of relevant experience in a laboratory setting
    Hands-on experience in cloning and assembly of DNA and growth of microbes
    Use of DNA design software, word processing, data collaboration tools, presentation and spreadsheet applications, and familiarity with concepts in statistics and tools to analyze data
    Strong organizational, time management, record keeping, and prioritization skills
    Independent, hard-working, and highly self-motivated
    Excellent communication and interpersonal relationship skills
    Ability to maintain a positive attitude when faced with challenges
    Desire to work in a team and assist other team members
    Awareness of safety issues in the work environment

    Preferred/Valued

    Experience with gas fermentation
    Experience in biotech industry
    Experience with metabolic engineering
    Experience different cloning methods such as Gibson or Golden Gate assembly and genetic tools such as CRISPR and homologous recombination
    Experience working with genetic and metabolic pathway databases and DNA design software
    Experience working with anaerobic microbes
    Strong computer literacy, including Office, SharePoint; Experience in scripting and working with large datasets is preferred
    Ability to conduct research independently and manage multiple tasks in parallel

    Physical Requirements

    Prolonged periods of sitting at a desk and working on a computer
    Prolonged periods of standing in the lab

    Work Environment

    HQ in Skokie, IL

     

    Apply HERE

  • ORISE fellow (Bachelor’s or Master’s level) – Biomanufacturing

    The Department of Defense (DoD) is offering a post-graduate internship at the U.S. Army Combat Capabilities Development Command – Chemical Biological Center (DEVCOM-CBC) within The Research and Technology (R&T) Directorate.

    As an ORISE participant, you will join a community of scientists and researchers in an effort to support The Research and Technology (R&T) Directorate within CCDC Chemical Biological Center, with a mission to provide integrated science and technology solutions to identify and mitigate novel and emerging chemical and biological threats.

    Why should I apply?
    Under the guidance of a mentor, you will gain hands-on experience to complement your education and support your academic and professional goals. During your appointment, you will participate in the configuration and operation of DEVCOM-CBC’s Biomanufacturing process development pilot plant. You will also have an opportunity to gain experience in fermentation, downstream processing, and biochemical analysis of the products of organisms modified using the techniques of synthetic biology at varying stages of purification and manufacture.

    Along the way, you will collaborate with team personnel to design and implement biological and chemical engineering strategies for production of critical chemicals and precursors, gaining exposure to production techniques including but not limited to medium- and large-scale fermentation, centrifugation, tangential-flow filtration, chromatography, distillation, and/or extraction. Under the mentorship of DEVCOM-CBC senior scientists, you will learn analytical techniques which will include HPLC, LC-MS, and GC-MS.

    In addition, you will learn and engage in activities which include but are not limited to:

    Technoeconomic analyses
    Process safety analysis
    Risk assessment
    Hazard mitigation in large-scale process development

    Where will I be located?
    Aberdeen Proving Ground, Maryland

    What is the anticipated start date?
    The DEVCOM-CBC is ready to make appointments immediately. Exact start dates will be determined at the time of selection and in coordination with the selected candidate. Applications are reviewed on an ongoing basis and internships will be filled as qualified candidates are identified.

    Appointment Length
    This appointment is a twelve month research appointment, with the possibility to be renewed for additional research periods. Appointments may be extended depending on funding availability, project assignment, program rules, and availability of the participant.

    What are the benefits?
    You will receive a stipend to be determined by DEVCOM-CBC. Stipends are typically based on a participant’s academic standing, discipline, experience, and research facility location. Other benefits may include the following:

    Health Insurance Supplement. Participants are eligible to purchase health insurance through ORISE.
    Relocation Allowance
    Training and Travel Allowance
    Nature of Appointment

    You will not enter into an employee/employer relationship with ORISE, ORAU, DoD, or any other office or agency. Instead, you will be affiliated with ORISE for the administration of the appointment through the ORISE appointment letter and Terms of Appointment.

    About DEVCOM-CBC
    U.S. Army Combat Capabilities Development Command Chemical Biological Center (DEVCOM-CBC) conducts applied research and development for the detection and identification of chemical, biological, and explosive materials to provide next-generation detection capabilities to the Warfighter. The Research and Technology (R&T) Directorate operates within CCDC Chemical Biological Center, with a mission to provide integrated science and technology solutions to identify and mitigate novel and emerging chemical and biological threats. The R&T Directorate specializes in the research and development of innovative technological solutions to solve chemical and biological defense threats to our nation—both abroad and in the homeland.

    Beginning in 2021, DEVCOM-CBC’s Biotechnology Branch begins a 2-year modernization and expansion effort of its 25,000 square-foot biomanufacturing research and development laboratory. Currently, the facility houses its bioproduction operations, featuring fermentation vessels up to 1000 liters capacity and downstream processing capability for bacterial and phage-based products. In the coming years, the facility will expand its capabilities to include downstream processing of critical specialty chemicals, precursor molecules, advanced bio-inspired polymer materials, and other military-relevant biologically derived products. At the completion of the modernization effort, the Biotechnology branch will serve the DoD as a pilot-scale biomanufacturing research and process development facility.

  • NRC Research Associate (postdoc or senior associate)

    We are seeking a candidate to work within the Chemical and Biological Center’s 20,000 square foot pilot-scale fermentation facility. DOD is realizing a surge in programs that are metabolically engineering yeast and bacteria to produce chemicals with unique properties. These are being developed into materials to support a wide variety of projects. Each requires scale up to 1500 liters, downstream processing, and characterization at labs located across the Edgewood campus. The successful candidate will interact with a multi-disciplinary team of material scientists, chemists, and biologists. The endeavour has regular interaction with academia and industry as part of a manufacturing consortium that spans the continetal United States. Good communication and problem solving skills are needed. Specific training will be provided with an opportunity for growth and international travel. Please contact for questions and background.

    POCs for questions about this opportunity are
    Peter Emanuel ([email protected])
    Henry Gibbons ([email protected])

  • NRC postdoc – Biomanufacturing

    Biomanufacturing is increasingly being viewed as an important tool to provide alternative and flexible domestic manufacturing capabilities for an agile supply chain. These technologies promise to boost the economy and secure domestic supply of important products, such as medicines or fuels. Traditionally, the vast majority of biomanufacturing efforts use cells in bioreactors to simultaneously grow and produce target products from cheap feedstocks. Cell-free systems are an emerging alternative in which cells are grown first and their expression and metabolic machinery harvested, followed by manufacturing of products using the extracted machinery. This approach has several advantages to cell-based manufacturing, including reduced sensitivity to products that inhibit growth, ability to produce products that are larger than the size of a cell, and rapid design and testing cycles that circumvent cloning. We are seeking NRC candidates that can support our efforts in cell-free biomanufacturing. Qualified candidates will have experience in cell-free systems, biomanufacturing, metabolic engineering, fermentation scale-up, microbiology, or synthetic biology; ideal candidates will have experience in both cell-free systems and biomanufacturing. Candidates will need to be flexible and willing to adapt to multiple roles within a large and evolving program.

    This project is a collaboration between U.S. Army and Northwestern University and offers opportunities for exchange among DOD, academic, and industry partners.

    Any questions can be directed to myself and the primary POC, Matt Lux ([email protected])

  • Lead Scientist, HTP Strain Screening

    Company Overview

    Mission-driven LanzaTech is a revolutionary solution to climate change, capturing pollution to clean our skies and oceans and create new high value products for a sustainable future. LanzaTech captures pollution to make fuels and chemicals, including sustainable jet fuel and products you use every day. The technology is like retrofitting a brewery onto an emission source like a steel mill or a landfill site, but instead of using sugars and yeast to make beer, pollution is converted by biology to fuels and chemicals! Imagine a day when your plane is powered by recycled emissions, when your yoga pants started life as pollution from a steel mill. This future is possible using LanzaTech technology.

    • 2021 Biofuels Digest #1 Hottest Company in Renewable Fuels, Chemicals & Biomaterials
    • 2020 Biofuels Digest #3 Best Company to work for in the Advanced Bioeconomy
    • 2020 Fast Company World Changing Company
    • 2020 CNBC #43 on Top 50 Disrupter Companies list
    • 2019 Chicago Innovation Awards Winner
    • 2019 Fortune Magazine – Change the World – Companies to watch
    • 2019 Kirkpatrick Chemical Engineering Achievement Award

    LanzaTech is committed to Diversity, Equity & Inclusion as part of our mission, culture & core values
    This position is open to candidates authorized to work in the United States on a full-time basis for any employer. LanzaTech is an Equal Employment Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, or veteran status.

    About the Role

    We are seeking an Automation Scientist to oversee LanzaTech’s biofoundry and high-throughput strain development pipeline, supporting the development of proprietary gas fermenting microbes for production of new molecules from waste gases. LanzaTech has designed, built and operates the world-first anaerobic biofoundry, a fully integrated platform of several automated modules with the capability of growing and manipulating thousands of anaerobic strains on gaseous feedstocks. A successful candidate will join LanzaTech’s Automation team and be a part of an effort pushing the boundaries of automation in context of anaerobic conditions and non-model organisms. The Automation team is a cross-functional team within LanzaTech’s Synthetic Biology group who support a variety of projects both within the group and across the company.

    Activities include automated, high-throughput bacterial transformation and culturing, strain quality control, and product screening as well as integration of new devices and emerging technologies. A successful candidate will also be expected to participate in data capture through both automated and semi-automated data management systems and the daily execution of high-throughput experiments and method development and optimization as well as coordination with various project teams.

    Duties & Responsibilities:

    Oversight and day-to-day operation of LanzaTech’s biofoundry and other automation systems including high-throughput culture, transformation, and screening of LanzaTech proprietary microbes;
    Definition, development and optimization of high-throughput workflows including integration of new instruments, emerging technologies and information systems;
    Aid in the further development of automated samples tracking and data capture by working the Computation Sciences and Computational Biology teams
    Use of laboratory information management systems (LIMS) and bioinformatics software tools and databases including data capture from high-throughput experiments and analysis of statistics and results against model predictions;
    Work in cross-functional setting including coordination with project team leaders;
    Attend seminars and participate in group and project meetings or lab activities as required.

    Requirements:

    M.Sc. or B.Sc. (or equivalent) in Synthetic Biology, Bioengineering, Biochemistry, Molecular Biology, Chemical Engineering or a related field.
    Minimum of 2 years’ experience in biotechnology industry, a biofoundry or a high-throughput environment with hand-on experience in high-throughput workflow development and/or execution, preferentially with high throughput microbial culture screening.
    Proficient in working with laboratory automation software and/or programming as well as statistics. Programming Hamilton liquid handing systems (Venus programming) and Thermo scheduling software (Momentum™) is highly preferred.
    Strong organizational, time management, and interpersonal skills. Ability to multi-task and keep track of multiple tasks and projects at the same time. High attention to detail and careful record keeping.
    Independent and highly self-motivated. Excellent communication skills and team-work oriented.
    Awareness of safety issues in the work environment.

    Desirable Skills:

    Proficiency in programming (e.g. Python)
    Experience with cell sorting
    Experience with microfluidics.
    Experience working with anaerobic microbes.

    Apply HERE

  • Bioinformatics Scientist

    Zymergen is hiring a Bioinformatics Scientist for enzyme optimization in strain engineering programs, via iterative cycles of Design-Build-Test-Learn, each supported by software-aided design and analysis software. The Bioinformatics Scientist will focus on working with colleagues to develop strategies and data-driven approaches to sampling enzyme diversity from metagenomic sequences, as well as enzyme performance optimization via protein engineering. The Scientist will join a team of Bioinformatics and Data Scientists working on metabolic pathway and strain design, genetic construct design, phenotype and genome analysis. Our aim is to improve the performance of microbes via multiple, iterative, rounds of genome engineering. In addition, the Scientist will be part of a larger technical team to develop best practices in designing strain edits and computer-aided learning for strain performance improvement.

    Responsibilities

    Explore state-of-the-art machine learning approaches to structure & function prediction from protein sequences, using our in-house LIMS datasets of sequences paired with enzyme performance data.
    Develop enzyme sequence selection strategies to sample enzyme sequence diversity contained in our proprietary metagenomics libraries, with 250M+ metagenomic sequences
    Evaluate available literature for a variety of metabolic enzymes in order to suggest strategies for metagenomic enzyme selection
    Prepare and present organized work plans and work product to multiple levels of management and diverse audiences
    Collaborate with academic and industrial business partners as needed to evaluate new technologies and/or to pursue joint ventures

    Qualifications/Experience

    • Master’s (or Bachelor’s with 2+ years ML experience) in one of the following relevant disciplines: Computer Science, Bioinformatics, Computational Biology, Biochemistry, Cell Biology,
    • Metabolic Engineering
    • Strong Python coding experience, including object-oriented programming
    • Experience with biological sequence handling (DNA/RNA/protein)
    • Experience with Tensorflow, PyTorch or related ML frameworks
    • Experience with SQL DB access via Python
    • Strong desire to collaborate and work with teams
    • Strong interpersonal skills. As a team, we work from a base of trust and assumption of good will

    Preferred Qualifications

    • Ph.D. in a relevant discipline
    • Rosetta, PyMol or related protein structural analysis
    • Experience with protein engineering
    • Experience in a metabolic engineering lab or company
    • Experience with ‘omics analysis (proteomics, genomics, metabolomics, etc.)
    • Experience with evolutionary and co-evolutionary analysis of protein sequences.
    • Experience with deep neural networks for biological sequences (e.g. Transformers, LSTMs, CNNs)

    Additional Details
    You should be familiar with (at least through coursework) and ideally, have applied current approaches to protein sequence modeling and structure / function prediction. Examples of these include Transformers (e.g. ESM-1b or MSA Transformer), LSTMs (e.g. UniRep), generative adversarial networks (GANs), autoencoders, convolutional neural networks (CNNs), as well as other deep neural network (DNN) architectures.

    You should have some experience in machine learning (ML) frameworks, including (but not limited to) PyTorch, Keras, Tensorflow, scikit-learn, caret (in R language), etc.

    You will need sufficient Python software engineering experience, above the level of a typical bioinformatics scientist (i.e. at the level of a Software Engineer I-II). This candidate should have a good understanding of object oriented programming in Python, as well as the use of integrated development environments (IDE’s), Git/GitHub, Python package management systems like pip, pipenv, conda and poetry, etc.

    Legal authorization to work in the U.S. is required. Zymergen may agree to sponsor an individual for an employment visa now or in the future if there is a shortage of individuals with particular skills for this job.

    In compliance with federal law, all persons hired will be required to verify identity and eligibility to work in the United States and to complete the required employment eligibility verification form upon hire.

  • Bioengineers and Scientists

    We are hiring bioengineers and scientists to help us accelerate the next-generation of medicines using cell-free!

    Who we are:
    SwiftScale Biologics uses our cell-free protein synthesis platform to enable (1) fast protein therapeutic development through to clinical batches and (2) synthesis of proteins that are very difficult to express in cells. Our mission is to enable our partners at top biotechs to advance dozens of novel therapeutics to the clinic. We have recently raised $20M from top investors, and are founded by field-leading professors in cell-free protein synthesis, Mike Jewett and Matt DeLisa.

    What we’re looking for:
    We are looking for someone who shares our goal of getting life-saving therapeutics to patients more quickly to join our tightly-knit team in South San Francisco, CA. You’ll expand the applications of cell-free technology by interfacing with key decision makers in major biotech companies and working with our team to deliver solutions using our growing cell-free platform.

    Key qualifications:
    •Experience with DNA design, molecular cloning, protein expression, purification, and characterization in high-throughput.

    •BS (+experience), MS, or PhD in Bioengineering, Biochemistry, Biomedical Engineering, Biotechnology, Chemical Engineering, or related fields.

    •Experience with automation and in vitro transcription translation or cell-free protein synthesis methods.

    •A drive to creatively solve problems by improving experimental workflows and developing new techniques in a fast-paced small company environment.

    Contact: [email protected]

  • Summer Teaching Assistant for BioBuilder

    About BioBuilder
    BioBuilder converts biological engineering research into hands-on laboratory content and novel problem-based learning modules that can be implemented by high school students and their teachers. Established from MIT’s Dept of BE and with National Science Foundation support, BioBuilder’s accessible curriculum has made a substantial impact on secondary and post-secondary life-science education, impacting teachers and students in nearly every state and more than 30 countries.

    Program Description
    For two weeks this summer, a group of international high school students will receive instruction in synthetic biology and biodesign principles from BioBuilder faculty. They will explore computational and creative thinking to analyze and present data. Teams will incorporate engineering principles into biodesigns of their own creation. Student deliverables will include a video presentation of their final project as well as a white paper summary of their research plan.

    Responsibilities
    The TA will assist students with topics in synthetic biology, computational and visualization techniques for biodesign, and the project design process. Work will require the TA to:
    ●Review and provide feedback to participants on assignments through Canvas platform;
    ●Meet virtually with teams to guide and assist participants through project design framework;
    ●Provide feedback on presentation and white paper drafts;
    ●Collaborate with Instructors on planning and curriculum as needed

    Qualifications
    ●Graduate student or post-doc in biological engineering
    ●Background in basic stats and coding (preferably RStudio)
    ●A strong desire to teach
    ●The ideal candidate will have some familiarity with BioBuilder curriculum and labs, or iGEM

    To apply: send an email expressing your interest in this position to BioBuilder’s Director of Operations, Jessie Klapper Elliott. [email protected]. Hire must complete a W-9 to receive stipend payment

  • POSTDOCTORAL POSITIONS ON SYNTHETIC TISSUE DEVELOPMENT

    The laboratory of Synthetic Biology and Regenerative Medicine at the University of Pittsburgh is looking for two postdoc candidates to join our research program on programming multicellular systems and designer organoids. The projects will use synthetic and systems biology to program the development of human tissues. The developed tissues are regenerative, scalable, with a high degree of robustness and functionality. We will also identify novel design principles governing human tissue morphogenesis and cooperative cellular behavior and develop new technologies for high content differentiation control through these projects. The model systems span from in vitro human pluripotent stem cells to mouse models. For more information, please see Cell Syst. 2021 20;12(1):41-55.e11 and Trends Biotechnology. 2018 Apr;36(4):415-429.
    The lab is located within the world-class Pittsburgh Liver Research Center (PLRC), home to a cutting-edge interdisciplinary research program on Liver diseases. We are also part of the McGowan Institute for Regenerative Medicine, Department of Pathology, and Department of Bioengineering. The candidate will have the opportunity to lead cutting edge highly interdisciplinary projects with collaborators at Carnegie Melon University, Massachusetts Institute of Technology, University of British Columbia, and Johns Hopkins University. You will have the opportunity to spend parts of your studies in the collaborators’ lab to enrich training experience and career development further. The candidate will engage with the stem cell and synthetic biology communities by presenting work at conferences and publishing high-impact papers and patents.
    Minimum/Basic Qualifications: MD and/or Ph.D. degree in a related field of study (bioengineering, molecular biology, and/or cell biology). Ability to perform standard molecular biology and mammalian cell culture techniques. Familiarity with synthetic and systems biology techniques, stem cell and organoids, in vivo mouse models, liver biology, and the hematopoietic system is a significant plus. A good track record of publication and ability to lead projects in fast pace environments is needed. Appointment: This position reports to Dr. Mo Ebrahimkhani and is situated at the PLRC and the university’s main campus. It is a full-time, benefits-eligible, fiscal appointment with a flexible start date. It comes with the possibility of annual renewal, dependent on available funding, performance, and the needs of the university. The salary follows the NIH Pay line scales for postdocs. Applications will be reviewed weekly until the search is closed. A background check is required for employment. The University of Pittsburgh is an equal opportunity/affirmative action employer committed to excellence through diversity.
    Women and minorities are encouraged to apply. Interested candidates should contact Dr. Mo Ebrahimkhani ([email protected]) with their CV, cover letter, and contact information of at least two referees included in the email.

  • POSTDOCTORAL POSITION ON GENE EDITING AND BIOREACTOR LIVER CHIP TECHNOLOGIES

    The laboratory of Dr. Samira Kiani at the University of Pittsburgh School of Medicine and Pittsburgh Liver Research Center is looking for an outstanding, highly motivated, and self-driven postdoc candidate to join a collaborative research program on gene editing and bioreactor liver chip technologies.
    Kiani lab is interested in using the designer DNA binding proteins such as Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and Zinc Finger proteins to develop novel gene and (epi)-gene-editing tools to address human diseases. Along with this notion, human microphysiological systems that can report on the immune response to and toxicity following the application of gene editors to human tissues are crucial in developing clinical gene editors. We work with the Liver on a Chip platform, a 3D perfusable system that allows us to develop and maintain human liver tissue over an extended period of time. We use patient-specific cells to assemble immune-competent liver tissues in this platform and analyze their response to gene editing events through high throughput assays such as transcriptomics, genomics, and Luminex assays. The goal is to develop biomarkers and predict response to gene editing in human tissues.
    We have contributed to the development of efficient CRISPR-based transcriptional repressors (Nat Methods. 2018 Aug;15(8):611-616), strategies to control gRNAs from RNA Pol II promoter (ACS Synth Biol. 2018 Aug 17;7(8):1929-1936) CRISPR layered control circuits and the immunogenicity of streptococcus Pyogenes CRISPR in human (https://doi.org/10.1101/360198).
    The lab is located within the world-class Pittsburgh Liver Research Center, home to a cutting-edge interdisciplinary research program on Liver diseases. The project is part of the Somatic Cell Genome Engineering Consortium, which provides a unique opportunity for interaction with the rest of the gene-editing and delivery community at MIT, U Berkley, University of Wisconsin, and elsewhere.
    In this position, you will have the opportunity to lead a cutting-edge project on the application and delivery of CRISPR in human liver Chip bioreactor platforms and participate in monthly meetings with the rest of the consortium members. You will be responsible for experimentally managing the project, contributing original ideas in carrying out the varied facets of the research endeavor, and expanding the lab operation, including writing grants.
    Minimum/Basic Qualifications: experience in any of the following: standard molecular biology techniques including PCR, gel electrophoresis, mammalian cell culture maintenance, viral vectors. Experience with qPCR, ELISAs, and confocal microscopy. MD or Ph.D. degree in a related field of study (genetics, microbiology, biology, bioengineering, molecular biology, and/or cell biology). A good track record of publications is required.
    Appointment: This position reports to Dr. Samira Kiani and is situated at the Pittsburgh Liver Research Center and the main campus of Pittsburgh University School of Medicine. It is a full-time, benefits-eligible, fiscal appointment with a flexible start date and the possibility of annual renewal, dependent on available funding, performance, and the needs of the University. The salary follows the NIH Pay line scales for postdocs. Applications will be reviewed weekly until the search is closed. A background check is required for employment. The University of Pittsburgh is an equal opportunity/affirmative action employer committed to excellence through diversity. Women and minorities are encouraged to apply.
    Interested candidates should contact Dr. Samira Kiani ([email protected]) with their CV, cover letter, and contact information of at least two referees included in the email.

  • NIST Cellular Engineering Group Postdoctoral Positions

    The NIST Cellular Engineering Group is seeking applicants for National Research Council Postdoctoral Research Associateships Program (https://www.nist.gov/iaao/academic-affairs-office/nist-nrc-postdoctoral-research-associateships-program).

    New measurement methods and tools are required for biology to emerge fully as an enabling, practical platform for engineering. The Cellular Engineering Group works to provide a foundation of measurement assurance to support the control and rational design of biological function. Through state-of-the-art synthetic biology, automation, and machine learning, the Group creates living measurement systems, such as cells, engineered to sense and respond in programmed ways. Importantly, building these systems both requires and advances meaningful quantitation of the effects of environmental context and evolution. The Group aims to advance fundamental understanding, improve predictability for design, ensure reproducibility and comparability, and facilitate scalability for real-world applications of engineered biological systems.

    We are seeking applicants interested in the following and related technical areas:

    • Experimental and theoretical research to advance rational design for engineering biology. Of particular interest is the development of new approaches to measure, understand, predict, and control information, learning, function, and evolution across the spatial and temporal scales of biological organization, although related ideas in other topics are welcome.
    • Absolute quantitation for engineering biology, in which mechanistic studies of gene expression are applied towards enabling more predictive engineering of living organisms. We welcome applicants with previous experience applying single-molecule techniques to understand energetics, thermodynamics, kinetics of protein-DNA interactions, and related phenomena, and connecting these measurements to organism fitness.
    • Cell-free systems for engineering biology, in which cell-free systems, engineering subcellular confinement, minimal life, synthetic cells, and related topics are investigated towards control and rational design of biological function.
    • Automation for engineering biology, in which methods are developed to create a measurement infrastructure for engineering biology. Collaborating with a multidisciplinary team, automation methods will be demonstrated to facilitate rapid and flexible adoption of automation systems for a variety of biological measurements.

    We are seeking independent, articulate, and motivated individuals with backgrounds across the physical, engineering, chemical, and biological disciplines to contribute to our collaborative, interdisciplinary Team. Facilities include state of the art systems for automated microbial engineering, culture, and measurement, single-molecule fluorescence microscopy, and cleanroom fabrication and characterization.

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