Joint Genome Institute

Lawrence Berkeley National Laboratory – Berkeley, California
Company size: 285
Anticipated number of internship positions: 1

Opportunity for remote/virtual internship in 2022: Yes

jgi.doe.gov

About JGI

The Department of Energy (DOE) Joint Genome Institute (JGI), a national user facility located at Lawrence Berkeley National Laboratory, is committed to advancing genomics in support of DOE missions related to clean energy generation and environmental characterization and cleanup. The JGI provides integrated high-throughput sequencing, DNA synthesis, metabolomics and computational analysis to enable systems- based scientific approaches to these challenges. The JGI promotes a diverse and vibrant research community dedicated to enabling large-scale scientific advances in a collaborative, inclusive environment.

Internship Project Description

(Updated 12/03/2021)

The Joint Genome Institute strain engineering group adapts genetically recalcitrant organisms of relevance to DOE Mission to enable, for example, modularized expression of heterologous DNA or gene/genome editing capabilities.

The objective of this project is to analyze the vast amounts of genomic data housed at JGI to identify and extract regulatory elements that are conserved across various taxonomic distances. For example, promoter sequences that are conserved at the Family, or Order level, can significantly simplify engineering efforts by ensuring expression of genetic components across many organisms. The outcomes of this computational analysis will be utilized for future microbial strain engineering. If on-site access is possible, the project will include downstream DNA assembly and testing in different microbial hosts. By including the full Design–Build–Test–Learn (DBTL) cycle into a project, the candidate can utilize and learn both dry-lab and wet-lab skills.

The 2022 JGI EBRC summer internship experience is anticipated to take place onsite at the new Integrative Genomics Building (IGB) at Lawrence Berkeley National Laboratory (Berkeley Lab), pending the relaxation of the pandemic constraints. If limited site access conditions continue, the co-mentors will revisit the design of the experiments to accommodate an enriching virtual experience.